RNA Details
Disease Name
idiopathic pulmonary fibrosis
Tissue
lung
RNA Symbol
mir-154
RNA ID
RNA Type
miRNA
Alteration Pattern
up regulation
Species
homo sapiens
Detection Methods
qRT-PCR; Analysis of 14q32 cluster; Chromatin immunoprecipitation assay etc.
Target
NA
Pathway
Wnt/B-catenin signaling pathway
PubMed ID
Title
Profibrotic role of miR-154 in pulmonary fibrosis
Year
2012
Function
"43 Significantly up-regulated microRNAs were identified in IPF lungs and 24 of the 43 increased microRNAs were localized to the chromosome 14q32 microRNA cluster where there are multiple SMAD3-binding elements. TGF-B1 stimulation of normal human lung fibroblasts (NHLF) caused up-regulation of microRNAs on chr14q32 that were also increased in IPF lungs. Chromatin immunoprecipitation confirmed binding of SMAD3 to the putative promoter of miR-154. Mir-154 was increased in IPF fibroblasts, and transfection of NHLF with miR-154 caused significant increases in cell proliferation,migration and activation of the WNT pathway ."